smoothelin (Novus Biologicals)
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smoothelin
Smoothelin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smoothelin/Smoothelin+Antibody/pmc11654679-263-41-43
Average 92 stars, based on 5 article reviews
Smoothelin, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/smoothelin/Smoothelin+Antibody/pmc11654679-263-41-43
Average 92 stars, based on 5 article reviews
smoothelin - by Bioz Stars,
2026-09
92/100 stars
Images
1) Product Images from "Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix"
Article Title: Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix
Journal: Science Advances
doi: 10.1126/sciadv.adr3530
Figure Legend Snippet: ( A ) To visualize SMCs, transverse sections of whole cervices from days 12, 15, 17, and 19 gravid mice ( n ≥ 3) were immunofluoresently stained with specific markers for a contractile (MYH11 and SMTN) or synthetic phenotype (RBP1 and MYH10). Sections were also stained with CNN1 and α-SMA, which are markers of contractile smooth muscle and myofibroblasts. The grayscale bar (left) is a schematic representation of markers associated with a particular SMC phenotype. Green indicates positive immunostaining for each marker; blue indicates nuclei counterstained with 4′,6-diamidino-2-phenylindole (DAPI). ( B ) Control immunoglobulin G (IgG; left) was used as a control for the primary antibodies, and primary antibodies were omitted as controls for the secondary antibodies. Representative dual immunostaining of MYH10 (green) and MYH11 (red). Scale bars, 1000 μm; inset scale bars, 60 μm. Inset depicts high-power view of select regions (dashed box) of interest, which show longitudinal- and circumferentially oriented contractile SMCs, as well as synthetic SMCs in the broad subepithelial region and diffusely throughout the stroma.
Techniques Used: Staining, Immunostaining, Marker, Control
Related Articles
Incubation:Article Title: Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix Article Snippet: For immunofluorescent staining, sections were fixed with 2% paraformaldehyde (Electron Microscopy Sciences) for 20 min. After washing with 1× PBS, sections were incubated for 1 hour at room temperature using blocking solution (10% goat serum and 0.25% Triton X-100 in 1X PBS). .. Sections were incubated overnight at 4°C with blocking solution containing the following primary antibodies: calponin (1:200 dilution; Abcam, ab46794), MYH10 (1:50; Cell Signaling Technologies, 3404), MYH11 (1:125; Abcam, ab224804), RBP1 (1:100, Abcam ab154881), α-SMA (1:200 dilution; Cell Signaling Technologies, 19245), or Article Title: Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix. Article Snippet: For immunofluorescent staining, sections were fixed with 2% paraformaldehyde (Electron Microscopy Sciences) for 20 min. After washing with 1× PBS, sections were incubated for 1 hour at room temperature using blocking solution (10% goat serum and 0.25% Triton X- 100 in 1X PBS). .. Sections were incubated overnight at 4°C with blocking solution containing the following primary antibodies: calponin (1:200 dilution; Abcam, ab46794), MYH10 (1:50; Cell Signaling Technologies, 3404), MYH11 (1:125; Abcam, ab224804), RBP1 (1:100, Abcam ab154881), α- SMA (1:200 dilution; Cell Signaling Technologies, 19245), or Article Title: Mid-old cells are a potential target for anti-aging interventions in the elderly Article Snippet: Counterstaining of each stained slide was performed using Hematoxylin II (790-2208, Ventana Medical Sytems Inc.). .. For IF staining of FFPE tissue sections, the following primary antibodies were incubated overnight at 4 °C: SAA1, 1:50 (MAB30191, R&D Systems); p21 Waf1 , 1:100 (ab109520, Abcam); IL1β, 1:100 (ab9722, Abcam); SLIT2, 1:200 (PA5-31133, Invitrogen); p16 INK4A , predilution (805-4713, Roche); Vimentin, 1:400 (ab92547, Abcam); Vimentin, 1:100 (AF2105, R&D Systems); Blocking Assay:Article Title: Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix Article Snippet: For immunofluorescent staining, sections were fixed with 2% paraformaldehyde (Electron Microscopy Sciences) for 20 min. After washing with 1× PBS, sections were incubated for 1 hour at room temperature using blocking solution (10% goat serum and 0.25% Triton X-100 in 1X PBS). .. Sections were incubated overnight at 4°C with blocking solution containing the following primary antibodies: calponin (1:200 dilution; Abcam, ab46794), MYH10 (1:50; Cell Signaling Technologies, 3404), MYH11 (1:125; Abcam, ab224804), RBP1 (1:100, Abcam ab154881), α-SMA (1:200 dilution; Cell Signaling Technologies, 19245), or Article Title: Regional differences in three-dimensional fiber organization, smooth muscle cell phenotype, and contractility in the pregnant mouse cervix. Article Snippet: For immunofluorescent staining, sections were fixed with 2% paraformaldehyde (Electron Microscopy Sciences) for 20 min. After washing with 1× PBS, sections were incubated for 1 hour at room temperature using blocking solution (10% goat serum and 0.25% Triton X- 100 in 1X PBS). .. Sections were incubated overnight at 4°C with blocking solution containing the following primary antibodies: calponin (1:200 dilution; Abcam, ab46794), MYH10 (1:50; Cell Signaling Technologies, 3404), MYH11 (1:125; Abcam, ab224804), RBP1 (1:100, Abcam ab154881), α- SMA (1:200 dilution; Cell Signaling Technologies, 19245), or Staining:Article Title: Mid-old cells are a potential target for anti-aging interventions in the elderly Article Snippet: Counterstaining of each stained slide was performed using Hematoxylin II (790-2208, Ventana Medical Sytems Inc.). .. For IF staining of FFPE tissue sections, the following primary antibodies were incubated overnight at 4 °C: SAA1, 1:50 (MAB30191, R&D Systems); p21 Waf1 , 1:100 (ab109520, Abcam); IL1β, 1:100 (ab9722, Abcam); SLIT2, 1:200 (PA5-31133, Invitrogen); p16 INK4A , predilution (805-4713, Roche); Vimentin, 1:400 (ab92547, Abcam); Vimentin, 1:100 (AF2105, R&D Systems); Formalin-fixed Paraffin-Embedded:Article Title: Mid-old cells are a potential target for anti-aging interventions in the elderly Article Snippet: Counterstaining of each stained slide was performed using Hematoxylin II (790-2208, Ventana Medical Sytems Inc.). .. For IF staining of FFPE tissue sections, the following primary antibodies were incubated overnight at 4 °C: SAA1, 1:50 (MAB30191, R&D Systems); p21 Waf1 , 1:100 (ab109520, Abcam); IL1β, 1:100 (ab9722, Abcam); SLIT2, 1:200 (PA5-31133, Invitrogen); p16 INK4A , predilution (805-4713, Roche); Vimentin, 1:400 (ab92547, Abcam); Vimentin, 1:100 (AF2105, R&D Systems); |